References

“About M. Luteus.” Modifiedmluteus, nstawebdirector.wixsite.com/modifiedmluteus/about-m-luteus. 

“Ancient Biotechnology.” Science Learning Hub, www.sciencelearn.org.nz/resources/1204-ancient-biotechnology.

Austin, Harry P., et al. “Characterization and Engineering of a Plastic-Degrading Aromatic Polyesterase.” PNAS, National Academy of Sciences, 8 May 2018, www.pnas.org/content/115/19/E4350

Bornscheuer, U. (2020, January 30th). Email Interview. 

Carrington, Damian. “Scientists Accidentally Create Mutant Enzyme That Eats Plastic Bottles.” The Guardian, Guardian News and Media, 16 Apr. 2018, www.theguardian.com/environment/2018/apr/16/scientists-accidentally-create-mutant-enzyme-that-eats-plastic-bottles. 

“Cas9 Mechanism.” CRISPRCas9, sites.tufts.edu/crispr/crispr-mechanism/. 

“Chlamydomonas Reinhardtii Assembly and Gene Annotation.” Details - Chlamydomonas Reinhardtii - Ensembl Genomes 46, plants.ensembl.org/Chlamydomonas_reinhardtii/Info/Annotation/. 

“Chlamydomonas Reinhardtii Complete Mitochondrial Genome - Nucleotide - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, www.ncbi.nlm.nih.gov/nuccore/U03843.1

“Engineers Generate Electricity by Tapping into Algae Cells.” ScienceDaily, ScienceDaily, 19 Apr. 2010, www.sciencedaily.com/releases/2010/04/100413121336.htm. 

“Full Stack Genome Engineering.” Synthego, www.synthego.com/learn/genome-engineering-history. 

“Genetics.” MIT Department of Biology, biology.mit.edu/faculty-and-research/areas-of-research/genetics/.

 

Gibson, Daniel G., et al. “Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome.” Science, American Association for the Advancement of Science, 2 July 2010, science.sciencemag.org/content/329/5987/52. 

“GMO Facts.” Non, www.nongmoproject.org/gmo-facts/. 

“Ideonella Sakaiensis (ID 291493).” National Center for Biotechnology Information, U.S. National Library of Medicine, www.ncbi.nlm.nih.gov/bioproject/291493

“Ideonella Sakaiensis Strain 201-F6 16S Ribosomal RNA, Partial Sequence - Nucleotide - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, www.ncbi.nlm.nih.gov/nuccore/NR_151910.1.

 

“Introduction to the CRISPR/Cas9 System.” Takara Bio-Home, www.takarabio.com/learning-centers/gene-function/gene-editing/gene-editing-tools-and-information/introduction-to-the-crispr/cas9-system

Irfan, Umair. “The Race to Save the Planet from Plastic.” Vox, Vox, 15 May 2019, www.vox.com/the-highlight/2019/4/9/18274131/plastic-waste-pollution-bacteria-digestion 

Joe, et al. “How to Make a GMO.” Science in the News, 11 Aug. 2015, sitn.hms.harvard.edu/flash/2015/how-to-make-a-gmo/.

 

Mathiesen, Karl. “Could a New Plastic-Eating Bacteria Help Combat This Pollution Scourge?” The Guardian, Guardian News and Media, 10 Mar. 2016, www.theguardian.com/environment/2016/mar/10/could-a-new-plastic-eating-bacteria-help-combat-this-pollution-scourge. 

“Methods of Genetic Engineering.” Mrlloyder, mrlloyder.weebly.com/methods-of-genetic-engineering.html. 

Palm, Gottfried J., et al. “Structure of the Plastic-Degrading Ideonella Sakaiensis MHETase Bound to a Substrate.” Nature News, Nature Publishing Group, 12 Apr. 2019, www.nature.com/articles/s41467-019-09326-3. 

Phytozome, phytozome.jgi.doe.gov/pz/portal.html#!info?alias=Org_Creinhardtii.

 

Radakovits, Randor, et al. “Genetic Engineering of Algae for Enhanced Biofuel Production.” Eukaryotic Cell, American Society for Microbiology Journals, 1 Apr. 2010, ec.asm.org/content/9/4/486.short. 

“Read ‘Genetically Engineered Crops: Experiences and Prospects’ at NAP.edu.” National Academies Press: OpenBook, www.nap.edu/read/23395/chapter/10#357

Staff, Creative Mechanisms. “Everything You Need to Know About The World's Most Useful Plastic (PET and Polyester).” , www.creativemechanisms.com/blog/everything-about-polyethylene-terephthalate-pet-polyester

The Editors of Encyclopaedia Britannica. “Genetic Engineering.” Encyclopædia Britannica, Encyclopædia Britannica, Inc., 19 Dec. 2019, www.britannica.com/science/genetic-engineering. 

“The GMO Gamble.” The Foodery, 5 Feb. 2018, fooderyboston.com/the-gmo-gamble/. 

Weber, G. (2020, January 30th). Email Interview. 

“What Are GM Crops and How Is It Done?: Royal Society.” What Are GM Crops and How Is It Done? | Royal Society, royalsociety.org/topics-policy/projects/gm-plants/what-is-gm-and-how-is-it-done/. 

Widyastuti, and Galuh. “Genetic Engineered Ideonella Sakaiensis Bacteria: A Solution of the Legendary Plastic Waste Problem.” SSRN, 20 June 2018, papers.ssrn.com/sol3/papers.cfm?abstract_id=3194556. 

Winchester, A.M. “Genetics.” Encyclopædia Britannica, Encyclopædia Britannica, Inc., 21 Nov. 2019, www.britannica.com/science/genetics. 

Yoshida, Shosuke, et al. “A Bacterium That Degrades and Assimilates Poly(Ethylene Terephthalate).” Science, American Association for the Advancement of Science, 11 Mar. 2016, science.sciencemag.org/content/351/6278/1196.

 

Yoshida, Shosuke, et al. “A Bacterium That Degrades and Assimilates Poly(Ethylene Terephthalate).” Science, American Association for the Advancement of Science, 11 Mar. 2016, science.sciencemag.org/content/351/6278/1196?ijkey=50ac4a7796f5a987be071a7eb392374313c4cab9&keytype2=tf_ipsecsha. 

Thank You To...

Our amazing coach and mentor for taking time out of their personal life to give us advice and feedback on our project.

Our wonderful teachers and administrators for supporting us through this competition and giving us this once in a lifetime opportunity.

The NSTA and Toshiba for creating ExploraVision. Without this competition we wouldn't have realized how fun and exciting competing in STEM could be.

And most importantly, our parents for allowing us to work before and after school, supporting us, and encouraging us to do the best work we can.