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References

“About M. Luteus.” Modifiedmluteus, nstawebdirector.wixsite.com/modifiedmluteus/about-m-luteus. 

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“Ancient Biotechnology.” Science Learning Hub, www.sciencelearn.org.nz/resources/1204-ancient-biotechnology.

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Austin, Harry P., et al. “Characterization and Engineering of a Plastic-Degrading Aromatic Polyesterase.” PNAS, National Academy of Sciences, 8 May 2018, www.pnas.org/content/115/19/E4350

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Bornscheuer, U. (2020, January 30th). Email Interview. 

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Carrington, Damian. “Scientists Accidentally Create Mutant Enzyme That Eats Plastic Bottles.” The Guardian, Guardian News and Media, 16 Apr. 2018, www.theguardian.com/environment/2018/apr/16/scientists-accidentally-create-mutant-enzyme-that-eats-plastic-bottles. 

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“Cas9 Mechanism.” CRISPRCas9, sites.tufts.edu/crispr/crispr-mechanism/. 

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“Chlamydomonas Reinhardtii Assembly and Gene Annotation.” Details - Chlamydomonas Reinhardtii - Ensembl Genomes 46, plants.ensembl.org/Chlamydomonas_reinhardtii/Info/Annotation/. 

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“Chlamydomonas Reinhardtii Complete Mitochondrial Genome - Nucleotide - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, www.ncbi.nlm.nih.gov/nuccore/U03843.1

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“Engineers Generate Electricity by Tapping into Algae Cells.” ScienceDaily, ScienceDaily, 19 Apr. 2010, www.sciencedaily.com/releases/2010/04/100413121336.htm. 

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“Full Stack Genome Engineering.” Synthego, www.synthego.com/learn/genome-engineering-history. 

“Genetics.” MIT Department of Biology, biology.mit.edu/faculty-and-research/areas-of-research/genetics/.

 

Gibson, Daniel G., et al. “Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome.” Science, American Association for the Advancement of Science, 2 July 2010, science.sciencemag.org/content/329/5987/52. 

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“GMO Facts.” Non, www.nongmoproject.org/gmo-facts/. 

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“Ideonella Sakaiensis (ID 291493).” National Center for Biotechnology Information, U.S. National Library of Medicine, www.ncbi.nlm.nih.gov/bioproject/291493

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“Ideonella Sakaiensis Strain 201-F6 16S Ribosomal RNA, Partial Sequence - Nucleotide - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, www.ncbi.nlm.nih.gov/nuccore/NR_151910.1.

 

“Introduction to the CRISPR/Cas9 System.” Takara Bio-Home, www.takarabio.com/learning-centers/gene-function/gene-editing/gene-editing-tools-and-information/introduction-to-the-crispr/cas9-system

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Irfan, Umair. “The Race to Save the Planet from Plastic.” Vox, Vox, 15 May 2019, www.vox.com/the-highlight/2019/4/9/18274131/plastic-waste-pollution-bacteria-digestion 

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Joe, et al. “How to Make a GMO.” Science in the News, 11 Aug. 2015, sitn.hms.harvard.edu/flash/2015/how-to-make-a-gmo/.

 

Mathiesen, Karl. “Could a New Plastic-Eating Bacteria Help Combat This Pollution Scourge?” The Guardian, Guardian News and Media, 10 Mar. 2016, www.theguardian.com/environment/2016/mar/10/could-a-new-plastic-eating-bacteria-help-combat-this-pollution-scourge. 

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“Methods of Genetic Engineering.” Mrlloyder, mrlloyder.weebly.com/methods-of-genetic-engineering.html. 

Palm, Gottfried J., et al. “Structure of the Plastic-Degrading Ideonella Sakaiensis MHETase Bound to a Substrate.” Nature News, Nature Publishing Group, 12 Apr. 2019, www.nature.com/articles/s41467-019-09326-3. 

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Weber, G. (2020, January 30th). Email Interview. 

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Widyastuti, and Galuh. “Genetic Engineered Ideonella Sakaiensis Bacteria: A Solution of the Legendary Plastic Waste Problem.” SSRN, 20 June 2018, papers.ssrn.com/sol3/papers.cfm?abstract_id=3194556. 

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Yoshida, Shosuke, et al. “A Bacterium That Degrades and Assimilates Poly(Ethylene Terephthalate).” Science, American Association for the Advancement of Science, 11 Mar. 2016, science.sciencemag.org/content/351/6278/1196.

 

Yoshida, Shosuke, et al. “A Bacterium That Degrades and Assimilates Poly(Ethylene Terephthalate).” Science, American Association for the Advancement of Science, 11 Mar. 2016, science.sciencemag.org/content/351/6278/1196?ijkey=50ac4a7796f5a987be071a7eb392374313c4cab9&keytype2=tf_ipsecsha. 

Thank You To...

Our amazing coach and mentor for taking time out of their personal life to give us advice and feedback on our project.

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Our wonderful teachers and administrators for supporting us through this competition and giving us this once in a lifetime opportunity.

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The NSTA and Toshiba for creating ExploraVision. Without this competition we wouldn't have realized how fun and exciting competing in STEM could be.

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And most importantly, our parents for allowing us to work before and after school, supporting us, and encouraging us to do the best work we can.

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